产品简要
文章摘录数: 1
产品信息
目录号 :
103729
产品名称 :
LSB-hsa-miR-758-5p
文章 :
doi | 10.1038/s41467-018-04575-0 |
id | 28192107 |
pubmed_id | 29934631 |
细菌的抗性 :
氨苄西林和卡那霉素
克隆 :
backbone | Low Sensor Backbone (LSB) | ||
backbone_mutation | |||
backbone_origin | |||
backbone_size | |||
promoter | |||
sequencing_primer_3 | |||
sequencing_primer_5 | |||
vector_types |
|
生长记录 :
Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.
生长株 :
Used to sense miRNA activity using fluorescence based tools. hsa-miR-758-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
起源 :
30
主要研究者 :
|
抗性标记物 :
855 |
标签 :
High Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
公司总部: 美国