产品简要
文章摘录数: 1
产品信息
目录号 :
103000
产品名称 :
pET22b_PfSSB
文章 :
| doi | PONE-D-17-36543 [pii] |
| id | 28193106 |
| pubmed_id | 29466468 |
细菌的抗性 :
氨苄西林
克隆 :
| backbone | pET22B | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
生长记录 :
The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, for subsequent labeling at the single, native cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~20-fold fluorescence increase on binding ssDNA with very high affinity. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77. Protocol See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233.
生长株 :
The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB
生长温度 :
BL21-DE3 pLysS for protein production
起源 :
37
主要研究者 :
|
质粒拷贝数 :
Synthetically produced by GenScript (The Biology CRO, U.S.A.)
抗性标记物 :
| 2835 |
标签 :
High Copy
更多信息或购买 :
公司信息
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
公司总部: 美国