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- domestic rabbit 多克隆
反应物种: 人类, 小鼠, 大鼠
应用: 免疫印迹
Figure 1. Western blot analysis of SP6 using anti-SP6 antibody (A08730-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,. Lane 2: human MCF-7 cell lysates, . Lane 3: rat spleen tissue lysates,. Lane 4: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP6 antigen affinity purified polyclonal antibody (Catalog # A08730-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP6 at approximately 45KD. The expected band size for SP6 is at 40KD.
规格: 100µg/vial
价格: 315美元
至产商
基因信息 - 小鼠 epiprofin
- 同义词:1110025J03Rik; AA591031; AI592962; Epfn; Klf14
- 描述:trans-acting transcription factor 6
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
产品类型
- epiprofin 抗体
- epiprofin 基因敲低
- epiprofin cDNA
- epiprofin 其他
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